Structures of NodZ α1,6-fucosyltransferase in complex with GDP and GDP-fucose
نویسندگان
چکیده
منابع مشابه
GDP-L-fucose transport in plants: The missing piece
The monosaccharide fucose is an important deoxyhexose occurring in plants, fungi and animals. Fucose is mainly found as a component of glycan structures such as Nand O-linked glycans, glycolipids and in cell wall polysaccharides such as fucoidan, xyloglucan, rhamnogalacturonan II. The biosynthesis of these glycan structures is mediated by glycosyltransferases and typically occurs within the end...
متن کاملThe crystal structure of human GDP-L-fucose synthase.
Human GDP-l-fucose synthase, also known as FX protein, synthesizes GDP-l-fucose from its substrate GDP-4-keto-6-deoxy-d-mannose. The reaction involves epimerization at both C-3 and C-5 followed by an NADPH-dependent reduction of the carbonyl at C-4. In this paper, the first crystal structure of human FX protein was determined at 2.37 Å resolution. The asymmetric unit of the crystal structure co...
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An improved procedure is described for the efficient and high-yield (76-91%) synthesis of nucleoside diphosphate sugars from the readily available nucleoside 5'-monophosphomorpholidate and sugar 1-phosphate in the presence of 1H-tetrazole. Comparative kinetic investigations by means of (31)P NMR spectroscopy with different additives (1,2,4-triazole, acetic acid, N-hydroxysuccinimide, 4-(dimethy...
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Notch (N) is a transmembrane receptor that mediates cell-cell interactions to determine many cell-fate decisions. N contains EGF-like repeats, many of which have an O-fucose glycan modification that regulates N-ligand binding. This modification requires GDP-L-fucose as a donor of fucose. The GDP-L-fucose biosynthetic pathways are well understood, including the de novo pathway, which depends on ...
متن کاملHigh-resolution structure of NodZ fucosyltransferase involved in the biosynthesis of the nodulation factor.
The fucosyltransferase NodZ is involved in the biosynthesis of the nodulation factor in nitrogen-fixing symbiotic bacteria. It catalyzes alpha1,6 transfer of l-fucose from GDP-fucose to the reducing residue of the synthesized Nod oligosaccharide. We present the structure of the NodZ protein from Bradyrhizobium expressed in Escherichia coli and crystallized in the presence of phosphate ions in t...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section D Biological Crystallography
سال: 2012
ISSN: 0907-4449
DOI: 10.1107/s0907444911053157